identification of candida species isolated from vulvovaginal candidiasis using pcr-rflp

نویسندگان

reyhaneh roshan

department of microbiology, karaj branch, islamic azad university, karaj, iran.سازمان اصلی تایید شده: دانشگاه آزاد اسلامی کرج (islamic azad university of karaj) azar sabokbar

department of microbiology, karaj branch, islamic azad university, karaj, iran.سازمان اصلی تایید شده: دانشگاه آزاد اسلامی کرج (islamic azad university of karaj) hamid badali

department of medical parasitology and mycology/molecular and cell biology research center; school of medicine, mazandaran university of medical sciences, sari, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (mazandaran university of medical sciences)

چکیده

vulvovaginal candidiasis (vvc) is a common disease among women worldwide, therefore, accurate and rapid diagnosis of causative agents based on molecular techniques utilizing amplification of target dna is highly recomendad for epidemiological purposes and for effective treatment. the aim of this study was to identify clinically candida species from vvc patients by restriction fragment length polymorphism (pcr-rflp). a total of 155 patients with suspicious symptoms of vvc were screened. candida strainsisolated from specimens initially were identified by phenotypic methods and confirmed by molecular approaches based on pcr-rflp. fifty one (34%) strains of candida were obtained from specimens collected from vvc patients. c. albicans was the most frequently isolated species (86.2%) followed by non-albicans, including c. glabrata (7.9%), c. kefir (3.9%), and c. tropicalis (1.9%). the restriction patterns of each candida species were perfectly specific. the identification of candida species in vvc due to developing antifungal resistance is very significant for appropriate treatment and to prevent the spread of vvc.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Identification of Candida species isolated from vulvovaginal candidiasis using PCR-RFLP

Vulvovaginal candidiasis (VVC) is a common disease among women worldwide, therefore, accurate and rapid diagnosis of causative agents based on molecular techniques utilizing amplification of target DNA is highly recomendad for epidemiological purposes and for effective treatment. The aim of this study was to identify clinically Candida species from VVC patients by restriction fragment length po...

متن کامل

Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal Candidiasis

Background: Vulvovaginal candidiasis (VVC) is an important health problem caused by Candida spp. The aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of non-albicans Candida isolates from VVC. Methods: Vaginal secretion samples were collected from 550 vaginitis patients at Sayyad Shirazi Medical and Educational Center of Gorgan (...

متن کامل

Comparison of Enzymatic Method Rapid Yeast Plus System with RFLP-PCR for Identification of Isolated Yeast from Vulvovaginal Candidiasis

Objective(s) To compare two identification methods, i.e., restriction fragment length polymorphism (RFLP)-PCR analysis and enzymatic method Rapid TM Yeast Plus System to identify different species causing vulvovaginal candidiasis (VVC). Materials and Methods Vaginal discharges of women who had attended the gynecology outpatient clinic of Mobini Hospital in Sabzevar, Iran were collected using...

متن کامل

Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal Candidiasis

Background Vulvovaginal candidiasis (VVC) is an important health problem caused by Candida spp. The aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of non-albicans Candida isolates from VVC. Methods Vaginal secretion samples were collected from 550 vaginitis patients at Sayyad Shirazi Medical and Educational Center of Gorgan (...

متن کامل

Use of PCR-RFLP and PCR-HWP1 for identification of Candida species isolated from cystic fibrosis patients

Background: Due to the predisposing conditions in patients with cystic fibrosis (CF) caused by defective mucociliary clearance facilitating colonization and invasion with Candida species has dramatically increased. Traditional methods for identifying problems are imminent and time-consuming. Therefore, molecular techniques utilizing amplification of target DNA provide quick and precise methods ...

متن کامل

Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR

BACKGROUND Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. METHODS 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sabouraud Dextrose Agar. The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fra...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of molecular and clinical microbiology

جلد ۴، شماره ۲، صفحات ۴۰۶-۴۱۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023